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SepaBean™ machine 2

Medium-Pressure Automated Flash Chromatography System

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If you want to find out more about the SepaBean Flash System Range or to book a demo, please get in touch with Chromatography Direct.

Overview

The SepaBean™ machine 2 is an advanced medium-pressure chromatography model with a higher flow rate of 300 mL/min and up to 500 psi (34.5 bar) backpressure tolerance. The system features a four-line binary gradient accommodating any two-solvent combination, with an option to add a third solvent as a modifier up to 15 %. 
 
 
SepaBean-Machine-T-Medium
  • Higher Flow Rate and Backpressure

    Achieves 300 mL/min flow rate and 500 psi 
    (34.5 bar) for faster and tougher separations.

  • Wireless Control and Operation

    Allows remote control via mobile devices, ideal for light-sensitive or isolated experiments.

  • Easy Flash Method Development

    Optimises and transfers separation conditions from HPLC and TLC for efficient, scalable purification.

  • Optional ELSD Detector

    Optional ELSD expands detection capabilities to cover a wider range of sample types, including non-UV-active compounds.

dual-piston-pump

Dual-Piston Pump

4-Lines

Four Solvent Lines

300-flow-rate

Maximum Flow Rate (mL/min) 

500-Pressure-Resistance

Pressure Resistance (psi)

machine 2 Specifications

  Specifications
User Interface 4.3 inches screen (≈ 10 cm)
Control Device Wireless operation through iPad®
Flow Rate Range 1 - 300 mL/min
Maximum Pressure 500 psi (34.5 bar)
Detectors • DAD 200 - 400 nm UV Detector
• DAD 200 - 800 nm UV/Vis Detector
• ELSD Universal Detector (optional)
Spectral Display Single, dual, or all-wavelengths
Pumping System high accuracy dual-piston pump
Number of Solvents Four (4) solvents binary with any combinations of two (2) solvents
Gradient Types Isocratic, linear, and step gradients
Column Sizes 4 g - 330 g, up to 1.6 kg with adapters
Sample Loading Capacity 4 mg - 160 g
Air Pump Module Included (to purge solvent residues)
Certification CE
21-CFR Part 11 Compliance The control software meets FDA
requirements for system safety
Collection Module LCD display for intelligent identification of test tube racks
Fraction Collection All, waste, threshold, slope, or time
Fraction Collector • 13, 15, 16, 18, or 25 mm tubes
• French square 250 or 500 mL bottle or large collection bottle (optional)
Safety Configuration Intelligent liquid and pressure alarm
Sample Injection Liquid and solid sampling
Smart Column Holder Suitable for 4 to 330 g flash columns
Dimensions (W x D x H) 44 x 46 x 63 cm
(17.3 x 18.1 x 24.8 in)
Weight 48 kg (106 lbs)

SepaBean Models

Large Scale Option up to 1000mL/min SepaBean machine L >>

Sepabean-machine-u

SepaBean machine U

Entry-level model designed to handle everyday separation and purification tasks.

Entry Level
SepaBean-Machnie-T

SepaBean machine T

Best-selling model, offering a highly cost-effective solution for efficient chromatography.

Cost Effective (Best Seller)
SepaBean-Standard

SepaBean machine

The standard version featuring a high pressure mixing.

Standard version
SepaBean-Machine-2

SepaBean machine 2

Medium-pressure model with a flow rate up to 300 mL/min and 500 psi (34.5 bar) tolerance.

Medium Pressure

SepaBean Brochure

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Application Notes

Application Notes using SepaBean

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Want to know more

If you want to find out more about the SepaBean instrument range, please get in touch with Chromatography Direct.