SepaBean™ Machine T
Flash Chromatography System
See the range
If you want to find out more about the SepaBean Flash System Range or to book a demo, please get in touch with Chromatography Direct.
Overview
The SepaBean™ machine T, our best-selling model, offers all the features of the SepaBean™ App, including wireless control and operation. This cost-effective system features a four-line binary gradient that supports any two-solvent combination, with the option to add a third solvent as a modifier (up to 15 %). An optional ELSD detector is available to expand detection capabilities to a broader range of sample types.-
Cost Effective Solution
Provides high-quality purification solutions within budget.
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Wireless Control and Operation
Allows remote control via mobile devices, ideal for light-sensitive or isolated experiments.
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Easy Flash Method Development
Optimises and transfers separation conditions from HPLC and TLC for efficient, scalable purification.
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Optional ELSD Detector
Optional ELSD expands detection capabilities to cover a wider range of sample types, including non-UV-active compounds.

Maintenance Free Pump

Four Solvent Lines

Maximum Flow Rate (mL/min) & Pressure Resistance (psi)
machine U Specifications
Specifications | |
User Interface | 4.3 inches screen (≈ 10 cm) |
Control Device | Wireless operation through iPad® |
Flow Rate Range | 1 - 100 mL/min |
Maximum Pressure | 200 psi (13.8 bar) |
Detectors | • DAD 200 - 400 nm UV Detector • DAD 200 - 800 nm UV/Vis Detector • ELSD Universal Detector (optional) |
Spectral Display | Single, dual, or all-wavelengths |
Pumping System | Maintenance free ceramic pump |
Number of Solvents | Four (4) solvents in binary mode with any combination of two (2) solvents, plus a third (3rd) solvent as a modifier |
Gradient Types | Isocratic, linear, and step gradients |
Column Sizes | 4 g - 330 g, up to 1.6 kg with adapters |
Sample Loading Capacity | 4 mg - 160 g |
Air Pump Module | Included (to purge solvent residues) |
Certification | CE, cTUVus |
21-CFR Part 11 Compliance | The control software meets FDA requirements for system safety |
Collection Module | LCD display for intelligent identification of test tube racks |
Fraction Collection | All, waste, threshold, slope, or time |
Fraction Collector | • 13, 15, 16, 18, or 25 mm tubes • French square 250 or 500 mL bottle or large collection bottle (optional) |
Safety Configuration | Intelligent liquid and pressure alarm |
Sample Injection | Liquid and solid sampling |
Smart Column Holder | Suitable for 4 to 330 g flash columns |
Dimensions | 40 x 40 x 62 cm (15.7 x 15.7 x 24.4 in) |
Weight | 39.2 Kg (86 lbs) |
SepaBean Models
Large Scale Option up to 1000mL/min SepaBean machine L >>

SepaBean Machine U
Entry-level model designed to handle everyday separation and purification tasks.

SepaBean Machine T
Best-selling model, offering a highly cost-effective solution for efficient chromatography.

SepaBean Standard
The standard version featuring a high pressure mixing.

SepaBean Machine 2
Medium-pressure model with a flow rate up to 300 mL/min and 500 psi (34.5 bar) tolerance.