SepaBean™ Machine U
Entry Level Automated Flash Chromatography System
See the range
If you want to find out more about the SepaBean Flash System Range or to book a demo, please get in touch with Chromatography Direct.
SepaBean machine U Overview
The SepaBean™ machine U, an entry-level model, offers an intuitive and versatile solution for chromatography with the full capabilities of SepaBean™ control software. Designed to handle everyday separation and purification tasks, this affordable system efficiently meets the demands of both normal and reversed phase separations, providing reliable performance for users of all experience levels.-
Cost Effective Solution
Provides high-quality purification solutions within budget.
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Wireless Control and Operation
Allows remote control via mobile devices, ideal for light-sensitive or isolated experiments.
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Easy Flash Method Development
Optimises and transfers separation conditions from HPLC and TLC for efficient, scalable purification.
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Optional ELSD Detector
Optional ELSD expands detection capabilities to cover a wider range of sample types, including non-UV-active compounds.

Maintenance Free Pump

Two Solvent Lines

Model U100
Maximum Flow Rate (mL/min) & Pressure Resistance (psi)

Model U200
Maximum Flow Rate (mL/min) & Pressure Resistance (psi)
machine U Specifications
Specifications | |
User Interface | 4.3 inches screen (≈ 10 cm) |
Control Device | Wireless operation through iPad® |
Flow Rate Range | • Model U100: 1 - 100 mL/min • Model U200: 1 - 200 mL/min |
Maximum Pressure | • Model U100: 100 psi (6.9 bar) • Model U200: 200 psi (13.8 bar) |
Detectors | • DAD 200 - 400 nm UV Detector • DAD 200 - 800 nm UV/Vis Detector • ELSD Universal Detector (optional) |
Spectral Display | Single, dual, or all-wavelengths |
Pumping System | Maintenance free ceramic pump |
Number of Solvents | Binary gradient, two (2) solvents |
Gradient Types | Isocratic, linear, and step gradients |
Column Sizes | 4 g - 330 g, up to 1.6 kg with adapters |
Sample Loading Capacity | 4 mg - 160 g |
Air Pump Module | Included (to purge solvent residues) |
Certification | CE |
21-CFR Part 11 Compliance | The control software meets FDA requirements for system safety |
Collection Module | LCD display for intelligent identification of test tube racks |
Fraction Collection | All, waste, threshold, slope, or time |
Fraction Collector | • 13, 15, 16, 18, or 25 mm tubes • French square 250 or 500 mL bottle or large collection bottle (optional) |
Safety Configuration | Intelligent liquid and pressure alarm |
Sample Injection | Liquid and solid sampling |
Smart Column Holder | Suitable for 4 to 330 g flash columns |
Dimensions | (W x D x H) 44 x 46 x 54 cm (17.3 x 18.1 x 21.3 in) |
Weight | 30 kg (66 lbs) |
SepaBean (machine) Models
Large Scale Option up to 1000mL/min SepaBean machine L >>

SepaBean Machine U
Entry-level model designed to handle everyday separation and purification tasks.

SepaBean Machine T
Best-selling model, offering a highly cost-effective solution for efficient chromatography.

SepaBean Standard
The standard version featuring a high pressure mixing.

SepaBean Machine 2
Medium-pressure model with a flow rate up to 300 mL/min and 500 psi (34.5 bar) tolerance.